#THIELICKE PIV IMAGEJ MANUAL#

The Rho GTPase-activating proteins RGA-3 and RGA-4 are required to set the initial size of PAR domains in Caenorhabditis elegans one-cell embryos. Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Long-term imaging of Caenorhabditis elegans using nanoparticle-mediated immobilization. PIVlab - Towards User-friendly, Affordable and Accurate Digital Particle Image Velocimetry in MATLAB.

Endrov: an integrated platform for image analysis. G., Johansson, A., Johansson, D., Bürglin, T. Computer visualization of three-dimensional image data using IMOD. A function for the midbody remnant in embryonic patterning. Coupling of rotational cortical flow, asymmetric midbody positioning, and spindle rotation mediates dorsoventral axis formation in C. Apical constriction drives tissue-scale hydrodynamic flow to mediate cell elongation.

He, B., Doubrovinski, K., Polyakov, O., Wieschaus, E.

Fast, accurate reconstruction of cell lineages from large-scale fluorescence microscopy data. De novo inference of systems-level mechanistic models of development from live-imaging-based phenotype analysis.

Du, Z., Santella, A., He, F., Tiongson, M., Bao, Z.

Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light.

Schrödel, T., Prevedel, R., Aumayr, K., Zimmer, M., Vaziri, A.

Complete kinetochore tracking reveals error-prone homologous chromosome biorientation in mammalian oocytes. Cell lineage reconstruction of early zebrafish embryos using label-free nonlinear microscopy. Imaging plant growth in 4D: robust tissue reconstruction and lineaging at cell resolution.

Fernandez, R., Das, P., Mirabet, V., Moscardi, E., Traas, J., Verdeil, J.

Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis.

Cell lineage analysis in ascidian embryos by intracellular injection of a tracer enzyme. Tissue-specific cell lineages originate in the gastrula of the zebrafish. The embryonic cell lineage of the nematode Caenorhabditis elegans. It is discussed how these methods can also be deployed to quantitatively capture other developmental processes in different models, e.g., cell tracking and lineage tracing, tracking of vesicle flow.

#THIELICKE PIV IMAGEJ MANUAL#

A reconstruction of a 3D cell-shape model using the modelling software IMOD, manual tracking of fluorescently-labeled subcellular structures using the multi-purpose image analysis program Endrov, and an analysis of cortical contractile flow using PIVlab (Time-Resolved Digital Particle Image Velocimetry Tool for MATLAB) are shown.

elegans strains available from the Caenorhabditis Genetics Center and on open-source software that can be easily implemented in any laboratory independently of the microscopy system used. The methods presented are all based on C. elegans animals for short- and long-term time-lapse microscopy and methods for tracking and quantification of developmental processes. Here protocols are presented for preparing embryos and adult C. Quantitatively capturing developmental processes is crucial to derive mechanistic models and key to identify and describe mutant phenotypes.

Thielicke piv imagej